{"id":544,"date":"2024-07-05T17:20:16","date_gmt":"2024-07-05T17:20:16","guid":{"rendered":"https:\/\/diyprp.com\/?p=544"},"modified":"2025-06-23T13:44:01","modified_gmt":"2025-06-23T13:44:01","slug":"lp-prp-preparation-analysis","status":"publish","type":"post","link":"https:\/\/diyprp.com\/en\/lp-prp-preparation-analysis\/","title":{"rendered":"LP-PRP Preparation &amp; Analysis"},"content":{"rendered":"\n<div class=\"wp-block-group has-global-padding is-layout-constrained wp-container-core-group-is-layout-e0082cf6 wp-block-group-is-layout-constrained\">\n<h3 class=\"wp-block-heading\"><strong>Introduction<\/strong><\/h3>\n\n\n\n<p>A leukocyte-poor PRP (LP-PRP) preparation was prepared using the NeutroFree PRP system&#8217;s protocol consisting of a large volume of whole blood collection and a double-spin method, accessing the buffy coat after the second spin to ensure higher platelet recovery.<\/p>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-group has-global-padding is-layout-constrained wp-block-group-is-layout-constrained\">\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"How to Prepare High-Quality LP-PRP with the NeutroFree PRP System | DIYPRP Protocol Overview\" width=\"500\" height=\"281\" src=\"https:\/\/www.youtube.com\/embed\/myJ45lMPxkU?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<\/div>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>Process Overview<\/strong><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>Process Steps<\/strong><\/h3>\n\n\n\n<ol class=\"wp-block-list\">\n<li><strong>Whole Blood Collection:<\/strong>\n<ul class=\"wp-block-list\">\n<li>ACD-A anticoagulant is used in a 1:9 ratio for the collection. Two 60 mL syringes are each filled with 3 mL of ACD-A anticoagulant. For more information about ACD-A and its formulation, read our post <a href=\"https:\/\/diyprp.com\/acda-anticoagulant\/\"><strong>here<\/strong><\/a>.<\/li>\n\n\n\n<li>27 mL of whole blood is then collected into each of the two 60 mL syringes (30 mL of content in each).<\/li>\n\n\n\n<li>One syringe collected 28 mL of whole blood, and a 1 mL baseline sample was collected for hemogram analysis.<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong>First Spin:<\/strong>\n<ul class=\"wp-block-list\">\n<li>The first spin of the 60 mL syringes is an RCF of 1,250 for 20 minutes, calculated by an Rmax of 155 mm, measured to the surface of the internal plunger of the syringe. For the centrifuge used, this was an RPM of 2,680.<\/li>\n\n\n\n<li>After the first spin, the PPP layer is carefully aspirated but left remaining are a few mLs of plasma, ensuring that the buffy coat is not disturbed, and then a 1 mL sample of the aspirated PPP was collected for hemogram analysis.<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong>Buffy Coat Extraction:<\/strong>\n<ul class=\"wp-block-list\">\n<li>After the PPP aspiration, the erythrocyte\/buffy coat interface remains intact and ready for extraction.<\/li>\n\n\n\n<li>The remaining PPP and buffy coat are then extracted along with a small amount of the erythrocyte layer (10 mL) into a new 20 mL syringe, ensuring the entire buffy coat and remaining platelets are collected for the second spin.<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong>Second Spin:<\/strong>\n<ul class=\"wp-block-list\">\n<li>The second spin is performed with a 20 mL syringe with an RCF of 380 for 5 minutes, calculated by an Rmax of 142 mm, measured to the surface of the internal plunger of the syringe. For the centrifuge used, this was an RPM of 1,550.<\/li>\n\n\n\n<li>After the second spin, the plasma layer, now 5 mL of highly concentrated PRP, is aspirated, again stopping short of the erythrocyte interface to avoid dilution and collection of granulocytes.<\/li>\n\n\n\n<li>A 1 mL sample of the 5 mL of prepared LP-PRP is collected for hemogram analysis. \n<ul class=\"wp-block-list\">\n<li>If activation of the platelets is required, read through our post about <a href=\"https:\/\/diyprp.com\/prp-activation-methods-comparison-lpprp-vs-lrprp\/\"><strong>platelet activation methods<\/strong><\/a>.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ol>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"How to Prepare LP-PRP with Neutrophil Reduction | DIYPRP Syringe-based Concentration System\" width=\"500\" height=\"281\" src=\"https:\/\/www.youtube.com\/embed\/LQ_cxua4jXk?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-group has-global-padding is-layout-constrained wp-container-core-group-is-layout-e0082cf6 wp-block-group-is-layout-constrained\">\n<figure class=\"wp-block-gallery has-nested-images columns-3 is-cropped wp-block-gallery-1 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"752\" height=\"1024\" data-id=\"556\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/60-ml-first-spin-1-752x1024.png\" alt=\"\" class=\"wp-image-556\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/60-ml-first-spin-1-752x1024.png 752w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/60-ml-first-spin-1-220x300.png 220w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/60-ml-first-spin-1-768x1046.png 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/60-ml-first-spin-1-1128x1536.png 1128w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/60-ml-first-spin-1-1504x2048.png 1504w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/60-ml-first-spin-1-9x12.png 9w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/60-ml-first-spin-1.png 1880w\" sizes=\"(max-width: 752px) 100vw, 752px\" \/><figcaption class=\"wp-element-caption\">First spin.<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"1645\" height=\"2174\" data-id=\"558\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/first-spin-ppp-1.png\" alt=\"\" class=\"wp-image-558\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/first-spin-ppp-1.png 1645w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/first-spin-ppp-1-227x300.png 227w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/first-spin-ppp-1-775x1024.png 775w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/first-spin-ppp-1-768x1015.png 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/first-spin-ppp-1-1162x1536.png 1162w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/first-spin-ppp-1-1550x2048.png 1550w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/first-spin-ppp-1-9x12.png 9w\" sizes=\"(max-width: 1645px) 100vw, 1645px\" \/><figcaption class=\"wp-element-caption\">PPP<\/figcaption><\/figure>\n<\/figure>\n\n\n\n<figure class=\"wp-block-gallery has-nested-images columns-3 is-cropped wp-block-gallery-2 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"664\" height=\"1024\" data-id=\"553\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/ppp-extraction-664x1024.png\" alt=\"\" class=\"wp-image-553\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/ppp-extraction-664x1024.png 664w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/ppp-extraction-195x300.png 195w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/ppp-extraction-768x1184.png 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/ppp-extraction-996x1536.png 996w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/ppp-extraction-1328x2048.png 1328w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/ppp-extraction-8x12.png 8w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/ppp-extraction.png 1660w\" sizes=\"(max-width: 664px) 100vw, 664px\" \/><figcaption class=\"wp-element-caption\">PPP aspiration.<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"1642\" height=\"2560\" data-id=\"554\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/buffy-coat-extraction.png\" alt=\"\" class=\"wp-image-554\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/buffy-coat-extraction.png 1642w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/buffy-coat-extraction-192x300.png 192w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/buffy-coat-extraction-657x1024.png 657w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/buffy-coat-extraction-768x1197.png 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/buffy-coat-extraction-985x1536.png 985w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/buffy-coat-extraction-1313x2048.png 1313w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/buffy-coat-extraction-8x12.png 8w\" sizes=\"(max-width: 1642px) 100vw, 1642px\" \/><figcaption class=\"wp-element-caption\">Buffy coat extraction.<\/figcaption><\/figure>\n<\/figure>\n\n\n\n<figure class=\"wp-block-gallery has-nested-images columns-2 is-cropped wp-block-gallery-3 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"691\" height=\"1024\" data-id=\"559\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/prp-20ml-691x1024.png\" alt=\"\" class=\"wp-image-559\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/prp-20ml-691x1024.png 691w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/prp-20ml-203x300.png 203w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/prp-20ml-768x1138.png 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/prp-20ml-1037x1536.png 1037w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/prp-20ml-1382x2048.png 1382w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/prp-20ml-8x12.png 8w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/prp-20ml.png 1728w\" sizes=\"(max-width: 691px) 100vw, 691px\" \/><figcaption class=\"wp-element-caption\">Second spin.<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"569\" height=\"1024\" data-id=\"560\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/prp-prepared-569x1024.png\" alt=\"\" class=\"wp-image-560\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/prp-prepared-569x1024.png 569w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/prp-prepared-167x300.png 167w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/prp-prepared-768x1381.png 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/prp-prepared-854x1536.png 854w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/prp-prepared-1139x2048.png 1139w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/prp-prepared-7x12.png 7w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/prp-prepared.png 1423w\" sizes=\"(max-width: 569px) 100vw, 569px\" \/><figcaption class=\"wp-element-caption\">5 mL Prepared PRP &#8211; 1 mL Sample<\/figcaption><\/figure>\n<\/figure>\n<\/div>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-group has-global-padding is-layout-constrained wp-container-core-group-is-layout-7db9d80f wp-block-group-is-layout-constrained\" style=\"padding-right:0;padding-left:0\">\n<h4 class=\"wp-block-heading\"><strong>Hemogram Observations<\/strong><\/h4>\n\n\n\n<p><strong>PRP Observations<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Concentration factor of <strong>6.82x<\/strong> in a <strong>5 mL<\/strong> of prepared PRP.<\/li>\n\n\n\n<li>High platelet recovery by over <strong>56%<\/strong>.<\/li>\n\n\n\n<li>Over <strong>4.4 billion<\/strong> platelets to provide efficacious therapy.<\/li>\n\n\n\n<li>Erythrocyte reduction by nearly <strong>100%<\/strong>.<\/li>\n\n\n\n<li>Granulocyte reduction by nearly <strong>100%<\/strong>.<\/li>\n\n\n\n<li>Leukocytes (granulocytes, lymphocytes, monocytes) all significantly reduced by over <strong>90%<\/strong>.<\/li>\n<\/ul>\n\n\n\n<p><strong>PPP Observations<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Virtually <strong>100%<\/strong> of erythrocytes and leukocytes unsuspended from plasma.<\/li>\n\n\n\n<li>PPP is virtually <strong>devoid <\/strong>of cells.<\/li>\n\n\n\n<li>Low platelet loss of only <strong>8.3%<\/strong>.<\/li>\n<\/ul>\n\n\n\n<div class=\"wp-block-group has-global-padding is-layout-constrained wp-block-group-is-layout-constrained\">\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading\"><strong>Platelet Isolation Analysis<\/strong><\/h4>\n\n\n\n<p>The following images demonstrate the platelet isolation process from leukocytes. To generate the remaining RBC, WBC, and PPP sample picture below in images 3 and 4, the remains were homogenized again and spun with the same RCF and time that produced the PRP. What we can identify here is that the plasma is devoid of suspended platelets, as they&#8217;ve been successfully isolated into another syringe and confirms what remains in the buffy coat are the heaviest WBCs and the RBCs below.<\/p>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-group has-global-padding is-layout-constrained wp-container-core-group-is-layout-e0082cf6 wp-block-group-is-layout-constrained\">\n<figure class=\"wp-block-gallery has-nested-images columns-3 is-cropped wp-block-gallery-4 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"576\" height=\"1024\" data-id=\"750\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8003-576x1024.jpg\" alt=\"\" class=\"wp-image-750\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8003-576x1024.jpg 576w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8003-169x300.jpg 169w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8003-768x1365.jpg 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8003-864x1536.jpg 864w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8003-1152x2048.jpg 1152w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8003-7x12.jpg 7w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8003.jpg 1440w\" sizes=\"(max-width: 576px) 100vw, 576px\" \/><figcaption class=\"wp-element-caption\">1. 2nd Spin Pre-aspirated PRP<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"1474\" height=\"2560\" data-id=\"751\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8007-1.jpg\" alt=\"\" class=\"wp-image-751\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8007-1.jpg 1474w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8007-1-173x300.jpg 173w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8007-1-590x1024.jpg 590w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8007-1-768x1334.jpg 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8007-1-884x1536.jpg 884w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8007-1-1179x2048.jpg 1179w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8007-1-7x12.jpg 7w\" sizes=\"(max-width: 1474px) 100vw, 1474px\" \/><figcaption class=\"wp-element-caption\">2. Aspirated PRP<\/figcaption><\/figure>\n<\/figure>\n\n\n\n<figure class=\"wp-block-gallery has-nested-images columns-3 is-cropped wp-block-gallery-5 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"564\" height=\"1024\" data-id=\"752\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8010-1-564x1024.jpg\" alt=\"\" class=\"wp-image-752\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8010-1-564x1024.jpg 564w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8010-1-165x300.jpg 165w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8010-1-768x1395.jpg 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8010-1-845x1536.jpg 845w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8010-1-1127x2048.jpg 1127w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8010-1-7x12.jpg 7w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8010-1.jpg 1409w\" sizes=\"(max-width: 564px) 100vw, 564px\" \/><figcaption class=\"wp-element-caption\">3. Remaining RBCs and WBCs after PRP aspiration. The plasma is clear of platelets.<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"1262\" height=\"2560\" data-id=\"753\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8013-1.jpg\" alt=\"\" class=\"wp-image-753\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8013-1.jpg 1262w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8013-1-148x300.jpg 148w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8013-1-505x1024.jpg 505w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8013-1-768x1558.jpg 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8013-1-757x1536.jpg 757w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8013-1-1010x2048.jpg 1010w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8013-1-6x12.jpg 6w\" sizes=\"(max-width: 1262px) 100vw, 1262px\" \/><figcaption class=\"wp-element-caption\">4. Remaining RBCs and WBCs after PRP aspiration. The plasma is clear of platelets.<\/figcaption><\/figure>\n<\/figure>\n\n\n\n<figure class=\"wp-block-gallery has-nested-images columns-2 is-cropped wp-block-gallery-6 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"1024\" height=\"838\" data-id=\"754\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8014-1-1024x838.jpg\" alt=\"\" class=\"wp-image-754\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8014-1-1024x838.jpg 1024w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8014-1-300x245.jpg 300w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8014-1-768x628.jpg 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8014-1-1536x1257.jpg 1536w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8014-1-2048x1675.jpg 2048w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8014-1-15x12.jpg 15w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\">5. Remaining RBCs, WBCs, and PPP vs. extracted PRP. The platelets have been successfully extracted.<\/figcaption><\/figure>\n<\/figure>\n<\/div>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-group has-global-padding is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\"><strong>Materials<\/strong><\/h4>\n\n\n\n<p>The materials needed to prepare <strong>Leukocyte-Poor Platelet-Rich Plasma (LP-PRP)<\/strong> vary depending on whether post-centrifugation analysis (e.g., hemogram, cell counts) is required. For anyone seeking to validate their preparations through laboratory analysis, additional supplies such as hematology analyzers, vacutainers, and micropipettes are necessary. However, for straightforward clinical use without laboratory validation, the minimum required equipment is much simpler. The list of required items grows substantially in more advanced configurations, as seen below:<\/p>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-image aligncenter size-large\"><img decoding=\"async\" width=\"1024\" height=\"653\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5782-1-1024x653.jpg\" alt=\"\" class=\"wp-image-740\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5782-1-1024x653.jpg 1024w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5782-1-300x191.jpg 300w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5782-1-768x490.jpg 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5782-1-1536x979.jpg 1536w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5782-1-2048x1306.jpg 2048w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5782-1-18x12.jpg 18w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><strong>At its most basic<\/strong>, LP-PRP preparation only requires the following:<\/p>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-image aligncenter size-large is-resized\"><img decoding=\"async\" width=\"1024\" height=\"1012\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8122-1024x1012.png\" alt=\"\" class=\"wp-image-738\" style=\"width:470px;height:auto\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8122-1024x1012.png 1024w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8122-300x296.png 300w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8122-768x759.png 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8122-1536x1517.png 1536w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8122-12x12.png 12w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_8122.png 1899w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<p><strong>Materials:<\/strong><\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Sterile Gloves<\/li>\n\n\n\n<li>Sterile Wipe<\/li>\n\n\n\n<li>ACD-A Anticoagulant Solution<\/li>\n\n\n\n<li>25 x 0.70 mm (22G x 1) Needle<\/li>\n\n\n\n<li>60 mL Leur Lock Syringe<\/li>\n\n\n\n<li>60 mL Leur Lock Syringe<\/li>\n\n\n\n<li>Sterile Wipe<\/li>\n\n\n\n<li>20 x 0.80 mm (21G x 0.75) Butterfly Scalp Needle<\/li>\n\n\n\n<li>Three-way Stopcock<\/li>\n\n\n\n<li>Band-aid (Medium)<\/li>\n\n\n\n<li>Sterile Gauze<\/li>\n\n\n\n<li>Band-aid (Small)<\/li>\n\n\n\n<li>Leur Lock Cap<\/li>\n\n\n\n<li>Leur Lock Cap&nbsp;<\/li>\n\n\n\n<li>60 mL Leur Lock Syringe<\/li>\n\n\n\n<li>Three-way Stopcock<\/li>\n\n\n\n<li>Leur Lock Cap<\/li>\n\n\n\n<li>10 mL Leur Lock Syringe<\/li>\n\n\n\n<li>Leur Lock Cap<\/li>\n\n\n\n<li>10 mL Leur Lock Syringe<\/li>\n\n\n\n<li>Leur Lock Cap<\/li>\n\n\n\n<li>Leur Lock-to-Leur Lock Transfer Connector<\/li>\n\n\n\n<li>10 mL Leur Lock Syringe<\/li>\n\n\n\n<li>Leur Lock Cap<\/li>\n<\/ol>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<p><strong>Extra Items (Not pictured):<\/strong><\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Tourniquet<\/li>\n\n\n\n<li>Garden Shears<\/li>\n\n\n\n<li>Cup with tap water (for counterweights)<\/li>\n\n\n\n<li>Scale<\/li>\n\n\n\n<li>Bucket inserts<\/li>\n\n\n\n<li>Centrifuge<\/li>\n<\/ol>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-group has-global-padding is-layout-constrained wp-block-group-is-layout-constrained\">\n<h3 class=\"wp-block-heading\"><strong>Platelet Recovery &amp; Quantity vs Concentration Factor<\/strong><\/h3>\n\n\n\n<p><strong>Platelet Recovery &amp; Quantity<\/strong><\/p>\n\n\n\n<p>The percentage of platelets recovered from the whole blood and the final platelet count of the prepared PRP are more precise indicators of the concentration system&#8217;s therapeutic efficacy and concentration efficiency. Final concentration factor is an unreliable means for determining the viability of any PRP system, as the concentration factor is determined by final volume, which can be easily manipulated.<\/p>\n\n\n\n<p>Higher platelet counts in PRP preparations have been shown to enhance the healing of soft tissues and reduce recovery times in orthopedic and sports medicine applications. The therapeutic benefits of PRP are directly related to the quantity of platelets (the dosage) and the subsequent availability of growth factors at the site of injury.<\/p>\n\n\n\n<p><strong>Concentration Considerations<\/strong><\/p>\n\n\n\n<p>PRP concentration factors are determined by the quantity of platelets and the volume of the PRP preparation. A higher volume will dilute the final concentration factor. Prepared PRP volume is determined by the PPP left remaining after the first spin&#8217;s aspiration. This PRP preparation created 5 mL of LP-PRP with a platelet count of 4.435 billion platelets leading to a concentration factor of 6.82x. Possible volume preparations and concentration factors:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>5 mL of PRP<\/strong> (4.435 billion platelets) = <strong>6.82x<\/strong><\/li>\n\n\n\n<li><strong>4 mL of PRP<\/strong> (4.435 billion platelets) = <strong>8.53x<\/strong><\/li>\n\n\n\n<li><strong>3 mL of PRP<\/strong> (4.435 billion platelets) = <strong>11.37x<\/strong><\/li>\n\n\n\n<li><strong>2 mL of PRP<\/strong> (4.435 billion platelets) = <strong>17.06x<\/strong><\/li>\n\n\n\n<li><strong>1 mL of PRP<\/strong> (4.435 billion platelets) = <strong>34.12x<\/strong><\/li>\n<\/ul>\n\n\n\n<p>As can be seen, the concentration factor may not be as important as the overall platelet count.<\/p>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-group has-global-padding is-layout-constrained wp-block-group-is-layout-constrained\">\n<h3 class=\"wp-block-heading\"><strong>Why Leukocyte-poor PRP?<\/strong><\/h3>\n\n\n\n<p>The NeutroFree PRP system&#8217;s primary function is to create high-quality leukocyte-poor PRP (LP-PRP), which is superior for intra-articular applications where inflammation is counterproductive. Inflammation can exacerbate joint damage and hinder the healing process. Certain leukocytes, particularly neutrophils and macrophages, release cytokines like IL-1 and TNF-\u03b1, which contribute to inflammation and cartilage breakdown in joints. By reducing the leukocyte content, LP-PRP minimizes the release of these inflammatory cytokines, thereby promoting a more conducive environment for tissue repair and regeneration. This makes LP-PRP an optimal choice for treating joint conditions, such as osteoarthritis, where controlling inflammation is crucial for effective healing.<\/p>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>Benefits of the NeutroFree PRP System<\/strong><\/h3>\n\n\n\n<p><strong>Low Cost<\/strong><\/p>\n\n\n\n<p>An interesting metric to consider when evaluating any PRP concentration system is the cost per billion platelets. A single preparation of PRP using this system costs approximately $16 USD. The prepared PRP has a dosage of 4.435 billion platelets making the cost-per-billion-platelets $3.61 USD.<\/p>\n\n\n\n<p><strong>High Platelet Recovery<\/strong><\/p>\n\n\n\n<p>Due to the system&#8217;s protocol and use of syringes, a consistently high platelet recovery of over 50% is possible.<\/p>\n\n\n\n<p><strong>Consistent and Precise Platelet Collection<\/strong><\/p>\n\n\n\n<p>Avoiding the use of needles for platelet collection, and relying on the precision of plungers for uniform platelet selection, the NeutroFree PRP system is able to effectively isolate the platelets from undesirable leukocytes.<\/p>\n\n\n\n<p><strong>Avoidance of Mechanical Activation of Platelets<\/strong><\/p>\n\n\n\n<p>By concentrating the platelets into the buffy coat and accessing them after a second spin, we can avoid the concentration of a platelet pellet that inadvertently leads to the activation of some platelets via mechanical forces from spinning.<\/p>\n\n\n\n<div class=\"wp-block-group has-global-padding is-layout-constrained wp-block-group-is-layout-constrained\">\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading\"><strong>Additional LP-PRP Preparation Images<\/strong><\/h4>\n\n\n\n<p>Additional images demonstrating the system&#8217;s performance.<\/p>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-group has-global-padding is-layout-constrained wp-container-core-group-is-layout-e0082cf6 wp-block-group-is-layout-constrained\">\n<figure class=\"wp-block-gallery has-nested-images columns-3 is-cropped wp-block-gallery-7 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"576\" height=\"1024\" data-id=\"756\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5837-576x1024.jpg\" alt=\"\" class=\"wp-image-756\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5837-576x1024.jpg 576w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5837-169x300.jpg 169w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5837-768x1365.jpg 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5837-864x1536.jpg 864w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5837-1152x2048.jpg 1152w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5837-7x12.jpg 7w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5837.jpg 1440w\" sizes=\"(max-width: 576px) 100vw, 576px\" \/><figcaption class=\"wp-element-caption\">Collected whole blood<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"1440\" height=\"2560\" data-id=\"757\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5838.jpg\" alt=\"\" class=\"wp-image-757\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5838.jpg 1440w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5838-169x300.jpg 169w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5838-576x1024.jpg 576w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5838-768x1365.jpg 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5838-864x1536.jpg 864w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5838-1152x2048.jpg 1152w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5838-7x12.jpg 7w\" sizes=\"(max-width: 1440px) 100vw, 1440px\" \/><figcaption class=\"wp-element-caption\">After the first spin<\/figcaption><\/figure>\n<\/figure>\n\n\n\n<figure class=\"wp-block-gallery has-nested-images columns-3 is-cropped wp-block-gallery-8 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"1024\" height=\"576\" data-id=\"758\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5846-1024x576.jpg\" alt=\"\" class=\"wp-image-758\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5846-1024x576.jpg 1024w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5846-300x169.jpg 300w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5846-768x432.jpg 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5846-1536x864.jpg 1536w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5846-2048x1152.jpg 2048w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5846-18x10.jpg 18w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\">PPP extraction<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"1440\" height=\"2560\" data-id=\"759\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5850.jpg\" alt=\"\" class=\"wp-image-759\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5850.jpg 1440w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5850-169x300.jpg 169w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5850-576x1024.jpg 576w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5850-768x1365.jpg 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5850-864x1536.jpg 864w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5850-1152x2048.jpg 1152w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5850-7x12.jpg 7w\" sizes=\"(max-width: 1440px) 100vw, 1440px\" \/><figcaption class=\"wp-element-caption\">Remaining whole blood with buffy coat and extracted PPP<\/figcaption><\/figure>\n<\/figure>\n\n\n\n<figure class=\"wp-block-gallery has-nested-images columns-2 is-cropped wp-block-gallery-9 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"576\" height=\"1024\" data-id=\"760\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5075-576x1024.jpg\" alt=\"\" class=\"wp-image-760\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5075-576x1024.jpg 576w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5075-169x300.jpg 169w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5075-768x1365.jpg 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5075-864x1536.jpg 864w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5075-1152x2048.jpg 1152w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5075-7x12.jpg 7w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5075.jpg 1440w\" sizes=\"(max-width: 576px) 100vw, 576px\" \/><figcaption class=\"wp-element-caption\">Supernatant PRP after second spin<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"1570\" height=\"2560\" data-id=\"761\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5076-1.jpg\" alt=\"\" class=\"wp-image-761\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5076-1.jpg 1570w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5076-1-184x300.jpg 184w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5076-1-628x1024.jpg 628w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5076-1-768x1252.jpg 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5076-1-942x1536.jpg 942w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5076-1-1256x2048.jpg 1256w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5076-1-7x12.jpg 7w\" sizes=\"(max-width: 1570px) 100vw, 1570px\" \/><figcaption class=\"wp-element-caption\">Extracted PRP<\/figcaption><\/figure>\n<\/figure>\n\n\n\n<figure class=\"wp-block-gallery has-nested-images columns-2 is-cropped wp-block-gallery-10 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"576\" height=\"1024\" data-id=\"762\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5044-576x1024.jpg\" alt=\"\" class=\"wp-image-762\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5044-576x1024.jpg 576w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5044-169x300.jpg 169w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5044-768x1365.jpg 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5044-864x1536.jpg 864w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5044-1152x2048.jpg 1152w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5044-7x12.jpg 7w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5044.jpg 1440w\" sizes=\"(max-width: 576px) 100vw, 576px\" \/><figcaption class=\"wp-element-caption\">Remaining WBCs\/Leukocytes after PRP extraction<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"1440\" height=\"2560\" data-id=\"763\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5038.jpg\" alt=\"\" class=\"wp-image-763\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5038.jpg 1440w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5038-169x300.jpg 169w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5038-576x1024.jpg 576w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5038-768x1365.jpg 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5038-864x1536.jpg 864w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5038-1152x2048.jpg 1152w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/IMG_5038-7x12.jpg 7w\" sizes=\"(max-width: 1440px) 100vw, 1440px\" \/><figcaption class=\"wp-element-caption\">Extracted PRP<\/figcaption><\/figure>\n<\/figure>\n<\/div>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<\/div>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-group has-global-padding is-layout-constrained wp-block-group-is-layout-constrained\">\n<h3 class=\"wp-block-heading\">Baseline Whole Blood<\/h3>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"1024\" height=\"522\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/03-07-sangue-total-1024x522.png\" alt=\"\" class=\"wp-image-545\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/03-07-sangue-total-1024x522.png 1024w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/03-07-sangue-total-300x153.png 300w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/03-07-sangue-total-768x391.png 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/03-07-sangue-total-1536x782.png 1536w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/03-07-sangue-total-2048x1043.png 2048w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/03-07-sangue-total-18x9.png 18w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\">Leukocyte-poor PRP (LP-PRP) &#8211; <strong>6.82x<\/strong><\/h3>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"1024\" height=\"518\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/03-07-prp-1024x518.png\" alt=\"\" class=\"wp-image-546\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/03-07-prp-1024x518.png 1024w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/03-07-prp-300x152.png 300w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/03-07-prp-768x389.png 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/03-07-prp-1536x777.png 1536w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/03-07-prp-2048x1037.png 2048w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/03-07-prp-18x9.png 18w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\">Platelet-poor Plasma (PPP)<\/h3>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"1024\" height=\"527\" src=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/03-07-ppp-1024x527.png\" alt=\"\" class=\"wp-image-547\" srcset=\"https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/03-07-ppp-1024x527.png 1024w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/03-07-ppp-300x154.png 300w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/03-07-ppp-768x395.png 768w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/03-07-ppp-1536x790.png 1536w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/03-07-ppp-2048x1054.png 2048w, https:\/\/diyprp.com\/wp-content\/uploads\/2024\/07\/03-07-ppp-18x9.png 18w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" style=\"margin-right:0;margin-left:0\">Data Table<\/h3>\n\n\n\n<figure class=\"wp-block-table\"><style>\n  table {\n    width: 100%;\n    border-collapse: collapse;\n  }\n  th, td {\n    border: 1px solid black;\n    padding: 8px;\n    text-align: center;\n  }\n  th {\n    background-color: #f2f2f2;\n  }\n  tr:nth-child(even) {\n    background-color: #f9f9f9;\n  }\n<\/style>\n\n<table>\n  <thead>\n    <tr>\n      <th>Parameter<\/th>\n      <th>Baseline Whole Blood<\/th>\n      <th>PRP<\/th>\n      <th>PPP<\/th>\n    <\/tr>\n  <\/thead>\n  <tbody>\n    <tr>\n      <td>RBC<\/td>\n      <td>3.67 (10^12\/L)<\/td>\n      <td>0.03 (10^12\/L)<\/td>\n      <td>0.00 (10^12\/L)<\/td>\n    <\/tr>\n    <tr>\n      <td>HCT<\/td>\n      <td>33.8%<\/td>\n      <td><\/td>\n      <td><\/td>\n    <\/tr>\n    <tr>\n      <td>MCV<\/td>\n      <td>92.1 fl<\/td>\n      <td><\/td>\n      <td><\/td>\n    <\/tr>\n    <tr>\n      <td>RDW%<\/td>\n      <td>14.7<\/td>\n      <td><\/td>\n      <td><\/td>\n    <\/tr>\n    <tr>\n      <td>RDWa<\/td>\n      <td>78.7 fl<\/td>\n      <td><\/td>\n      <td><\/td>\n    <\/tr>\n    <tr>\n      <td>HGR<\/td>\n      <td>10.2 g\/dL<\/td>\n      <td>0.2 g\/dL<\/td>\n      <td>0.0 g\/dL<\/td>\n    <\/tr>\n    <tr>\n      <td>MCH<\/td>\n      <td>27.8 pg<\/td>\n      <td><\/td>\n      <td><\/td>\n    <\/tr>\n    <tr>\n      <td>MCHC<\/td>\n      <td>30.2 g\/dL<\/td>\n      <td><\/td>\n      <td><\/td>\n    <\/tr>\n    <tr>\n      <td>PLT<\/td>\n      <td>130 (10^9\/L)<\/td>\n      <td>887 (10^9\/L)<\/td>\n      <td>13 (10^9\/L)<\/td>\n    <\/tr>\n    <tr>\n      <td>PCT<\/td>\n      <td>0.1%<\/td>\n      <td>0.64%<\/td>\n      <td><\/td>\n    <\/tr>\n    <tr>\n      <td>MPV<\/td>\n      <td>8.1 fl<\/td>\n      <td>7.3 fl<\/td>\n      <td><\/td>\n    <\/tr>\n    <tr>\n      <td>PDW<\/td>\n      <td>12.4 fl<\/td>\n      <td>11.0 fl<\/td>\n      <td><\/td>\n    <\/tr>\n    <tr>\n      <td>LPCR<\/td>\n      <td>19.2%<\/td>\n      <td>11.5%<\/td>\n      <td><\/td>\n    <\/tr>\n    <tr>\n      <td>WBC<\/td>\n      <td>3.3 (10^9\/L)<\/td>\n      <td>1.8 (10^9\/L)<\/td>\n      <td>0.0 (10^9\/L)<\/td>\n    <\/tr>\n    <tr>\n      <td>LYM<\/td>\n      <td>1.5 (45%)<\/td>\n      <td>1.6 (90.7%)<\/td>\n      <td><\/td>\n    <\/tr>\n    <tr>\n      <td>GRAN<\/td>\n      <td>1.5 (44.4%)<\/td>\n      <td>0.1 (5.6%)<\/td>\n      <td><\/td>\n    <\/tr>\n    <tr>\n      <td>MTD<\/td>\n      <td>0.3 (10.6%)<\/td>\n      <td>0.1 (3.7%)<\/td>\n      <td><\/td>\n    <\/tr>\n    <tr>\n      <td><strong>Total Platelets (in 10^9)<\/strong><\/td>\n      <td><strong>7.800<\/strong><\/td>\n      <td><strong>4.435<\/strong><\/td>\n      <td><strong>0.065<\/strong><\/td>\n    <\/tr>\n    <tr>\n      <td><strong>Recovery Percentage of Platelets<\/strong><\/td>\n      <td><strong><\/strong><\/td>\n      <td><strong>56.86%<\/strong><\/td>\n      <td><strong><\/strong><\/td>\n    <\/tr>\n    <tr>\n      <td><strong>Reduction % RBC<\/strong><\/td>\n      <td><strong><\/strong><\/td>\n      <td><strong>99.93%<\/strong><\/td>\n      <td><strong>100%<\/strong><\/td>\n    <\/tr>\n    <tr>\n      <td><strong>Reduction % WBC<\/strong><\/td>\n      <td><strong><\/strong><\/td>\n      <td><strong>95.45%<\/strong><\/td>\n      <td><strong>100%<\/strong><\/td>\n    <\/tr>\n    <tr>\n      <td><strong>Reduction % LYM<\/strong><\/td>\n      <td><strong><\/strong><\/td>\n      <td><strong>91.11%<\/strong><\/td>\n      <td><strong>100%<\/strong><\/td>\n    <\/tr>\n    <tr>\n      <td><strong>Reduction % GRAN<\/strong><\/td>\n      <td><strong><\/strong><\/td>\n      <td><strong>99.44%<\/strong><\/td>\n      <td><strong>100%<\/strong><\/td>\n    <\/tr>\n    <tr>\n      <td><strong>Reduction % MTD<\/strong><\/td>\n      <td><strong><\/strong><\/td>\n      <td><strong>97.22%<\/<\/strong><\/td>\n      <td><strong>100%<\/strong><\/td>\n    <\/tr>\n    <tr>\n      <td><strong>Recovery Percentage of WBC<\/strong><\/td>\n      <td><strong><\/strong><\/td>\n      <td><strong>4.55%<\/strong><\/td>\n      <td><strong>0%<\/strong><\/td>\n    <\/tr>\n    <tr>\n      <td><strong>Platelet Concentration<\/strong><\/td>\n      <td><strong><\/strong><\/td>\n      <td><strong>6.82x<\/strong><\/td>\n      <td><strong><\/strong><\/td>\n    <\/tr>\n  <\/tbody>\n<\/table>\n<\/figure>\n<\/div>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-group has-global-padding is-layout-constrained wp-block-group-is-layout-constrained\">\n<h2 class=\"wp-block-heading\">References<\/h2>\n\n\n\n<ol class=\"wp-block-list\">\n<li class=\"has-system-serif-font-family has-medium-font-size\">Mishra, A., et al. (2014). Platelet-rich plasma in orthopedic applications: evidence-based recommendations for treatment. <em>Journal of the American Academy of Orthopaedic Surgeons, 22<\/em>(18), 469-470.<\/li>\n\n\n\n<li class=\"has-system-serif-font-family has-medium-font-size\">Sundman, E. A., et al. (2014). The anti-inflammatory and matrix restorative mechanisms of platelet-rich plasma in osteoarthritis. <em>American Journal of Sports Medicine, 42<\/em>(1), 35-41.<\/li>\n\n\n\n<li class=\"has-system-serif-font-family has-medium-font-size\">Riboh, J. C., et al. (2016). Effect of leukocyte concentration on the efficacy of platelet-rich plasma in the treatment of knee osteoarthritis. <em>American Journal of Sports Medicine, 44<\/em>(3), 792-800.<\/li>\n<\/ol>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Using the NeutroFree PRP system, a leukocyte-poor PRP (LP-PRP) was prepared through a double-spin method, achieving a high concentration factor of 6.82x in a 5 mL sample. This process ensures over 4.4 billion platelets for effective therapy, with significant reductions in erythrocytes and leukocytes. The resulting PRP offers enhanced healing potential with minimal inflammation.<\/p>\n","protected":false},"author":1,"featured_media":754,"comment_status":"open","ping_status":"open","sticky":true,"template":"","format":"standard","meta":{"footnotes":""},"categories":[132,114],"tags":[125,128,126,129,122,131,120,118,121,116,117,119,106,101,115,127,130,123],"class_list":["post-544","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-featured","category-prp-preparation","tag-aplicacoes-intra-articulares","tag-cicatrizacao-de-articulacoes","tag-citocinas","tag-controle-de-inflamacao","tag-cytokines","tag-featured","tag-inflammation-control","tag-intra-articular-applications","tag-joint-healing","tag-leukocyte-poor-prp","tag-neutrofree-prp","tag-osteoarthritis-treatment","tag-plasma-rico-em-plaquetas","tag-platelet-rich-plasma","tag-prp","tag-prp-pobre-em-leucocitos","tag-regeneracao-de-tecidos","tag-tissue-regeneration"],"_links":{"self":[{"href":"https:\/\/diyprp.com\/en\/wp-json\/wp\/v2\/posts\/544","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/diyprp.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/diyprp.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/diyprp.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/diyprp.com\/en\/wp-json\/wp\/v2\/comments?post=544"}],"version-history":[{"count":34,"href":"https:\/\/diyprp.com\/en\/wp-json\/wp\/v2\/posts\/544\/revisions"}],"predecessor-version":[{"id":804,"href":"https:\/\/diyprp.com\/en\/wp-json\/wp\/v2\/posts\/544\/revisions\/804"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/diyprp.com\/en\/wp-json\/wp\/v2\/media\/754"}],"wp:attachment":[{"href":"https:\/\/diyprp.com\/en\/wp-json\/wp\/v2\/media?parent=544"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/diyprp.com\/en\/wp-json\/wp\/v2\/categories?post=544"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/diyprp.com\/en\/wp-json\/wp\/v2\/tags?post=544"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}