System Performance

Leukocyte-poor Preparation 26/06/2024

Baseline Whole Blood

PRP 1 – LP-PRP (more poor) – 7.3x

PRP 2 – LP-PRP (less poor) – 8.4x

Data Table

Parameter	Baseline Whole Blood	PRP 1 LP-PRP (more poor)	PRP 2 LP-PRP (less poor)
RBC	5.42	0.0	0.01
HCT	50.5%
MCV	93 fl
RDW%	14.6%
RDWa	80.3 fl
HGR	15.2 g/dL	0.1 g/dL	0.1 g/dL
MCH	28.1 pg
MCHC	30.2 g/dL
PLT	73 (10^9/L)	534 (10^9/L)	617 (10^9/L)
PCT	0.06%	0.33%	0.39%
MPV	8.5 fl	6.2 fl	6.4 fl
PDW	13.1 fl	9.6 fl	9.7 fl
LPCR	20.7%	5.4%	7.4%
WBC	5.9 (10^9/L)	0.1 (10^9/L)	0.8 (10^9/L)
LYM	2.5 (43%)		0.8 (92.8%)
GRAN	3.0 (51%)		0.0 (2.3%)
MTD	0.4 (6.0%)		0.0 (4.9%)
Reduction % RBC		100.0%	99.82%
Reduction % WBC		98.31%	86.44%
Reduction % LYM		100.0%	68.0%
Reduction % GRAN		100.0%	100.0%
Reduction % MTD		100.0%	100.0%
Concentration Score		7.32x	8.45x

Hemogram Observations

PRP 1 LP-PRP (more poor)

• Concentration factor of 7.32x.
• Erythrocyte reduction by nearly 100%.
• Granulocyte reduction by nearly 100%.
• Leukocytes (granulocytes, lymphocytes, monocytes) all significantly reduced by over 98%.

PRP 2 LP-PRP (less poor)

• Concentration factor of 8.45x.
• Erythrocyte reduction by nearly 100%.
• Granulocyte reduction by nearly 100%.
• Leukocytes (granulocytes, lymphocytes, monocytes) all significantly reduced by over 86%.

Leukocyte-poor PRP Preparation 04/07/2024

Baseline Whole Blood

Leukocyte-poor PRP (LP-PRP) – 6.82x

Platelet-poor Plasma (PPP)

Data Table

Parameter	Baseline Whole Blood	PRP	PPP
RBC	3.67 (10^12/L)	0.03 (10^12/L)	0.00 (10^12/L)
HCT	33.8%
MCV	92.1 fl
RDW%	14.7
RDWa	78.7 fl
HGR	10.2 g/dL	0.2 g/dL	0.0 g/dL
MCH	27.8 pg
MCHC	30.2 g/dL
PLT	130 (10^9/L)	887 (10^9/L)	13 (10^9/L)
PCT	0.1%	0.64%
MPV	8.1 fl	7.3 fl
PDW	12.4 fl	11.0 fl
LPCR	19.2%	11.5%
WBC	3.3 (10^9/L)	1.8 (10^9/L)	0.0 (10^9/L)
LYM	1.5 (45%)	1.6 (90.7%)
GRAN	1.5 (44.4%)	0.1 (5.6%)
MTD	0.3 (10.6%)	0.1 (3.7%)
Total Platelets (in 10^9)	7.800	4.435	0.065
Recovery Percentage of Platelets		56.86%
Reduction % RBC		99.93%	100%
Reduction % WBC		95.45%	100%
Reduction % LYM		91.11%	100%
Reduction % GRAN		99.44%	100%
Reduction % MTD		97.22%	100%
Recovery Percentage of WBC		4.55%	0%
Platelet Concentration		6.82x

Hemogram Observations

PRP Observations

• Concentration factor of 6.82x in a 5 mL of prepared PRP.
• High platelet recovery by over 56%.
• Over 4.4 billion platelets to provide efficacious therapy.
• Erythrocyte reduction by nearly 100%.
• Granulocyte reduction by nearly 100%.
• Leukocytes (granulocytes, lymphocytes, monocytes) all significantly reduced by over 90%.

PPP Observations

• Virtually 100% of erythrocytes and leukocytes unsuspended from plasma.
• PPP is virtually devoid of cells.
• Low platelet loss of only 8.3%.

Leukocyte-rich PRP Preparation 26/06/2024